제품

Helicobacter Pylori 항체 시약--일차 항체

Helicobacter Pylori 항체 시약-- 1 차 항체

양성 대조군:
tissue infected by H. Pylori
셀룰러 현지화:
H. Pylori
애플리케이션:
IHC-P
이차항체:
iVision™
사양/ml:
1、3、6、0.2（Concentrated）

지금 문의

제품 상세 정보

【Product name】

Helicobacter Pylori Antibody reagent--Primary Antibody

【Packing specification】

Code	Clone	Specifications
AP0133	polyclonal	0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml
AR0133	TLR0133	0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml

【Intended use】

For Research use only. Helicobacter Pylori Antibody reagent is intended for use to qualitatively identify Helicobacter Pylori by microscopy in sections of FFPE tissue using immunohistochemical detection system.

【Principle】

Helicobacter Pylori (H. Pylori) is an endemic helix-shaped Gram-negative bacterium that infected over half of the world’s population. Infection with H. Pylori is responsible for the majority of duodenal and gastric ulcers, and have been associated with increased risk of developing mucosa-associate lymphoid tissue (MALT) lymphoma, atrophic gastritis and gastric cancer. Antibody to H. pylori is useful for detecting the bacterial infection in gastric and duodenal epithelial cells. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody-primary antibody-antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen.

【Main components】

Immunoglobulin, antibody diluent

【Storage】

Store at 2～8℃ for 18 months。

【Sample requirements】

FFPE tissues are usually cut into sections as thin as 3～5μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.

【Protocol】

1. Sample preparation：Deparaffinize the slides in xylene Ⅰ, Ⅱ, Ⅲ for 5 minutes；Transfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2. Blocking：Block endogenous peroxidase activity by incubating sections in 3% H₂O₂ solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3. Antigen retrieval：Heat the EDTA Antigen retrieval buffer to 100℃. Then place the slides in the boiled buffer and continue to heat for 15～20 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4. Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing.

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DAB：Drain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin staining：Drain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. Dehydrate：Dehydrate the slides in 75%, 95%, 100% alcohols for 2 minutes. Dry the slides. Cover stained tissue with a coverslip using mounting medium.