제품

IHC 항체 -- 인간 Chorionic Gonadotropin

양성 대조군:
Placenta
셀룰러 현지화:
Cytoplasm
애플리케이션:
IHC-P
이차항체:
iVision™
사양/ml:
1、3、6、0.2（Concentrated）

지금 문의

제품 상세 정보

【Product name】

IHC Antibody -- Human Chorionic Gonadotropin

【Packing specification】

Code	Clone	Specifications
AM0130	ZSH17	0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml
AP0130	polyclonal	0.1ml, 0.2ml, 1ml, 1.5ml, 3ml, 6ml, 11ml, 30ml

【Intended use】

For Research use only. Human Chorionic Gonadotropin Antibody reagent is intended for use to qualitatively identify Human Chorionic Gonadotropin by microscopy in sections of FFPE tissue using immunohistochemical detection system.

【Principle】

Human Chorionic Gonadotropin (HCG) is a hormone synthesized by syncytiotrophoblasts and belongs to a group of glycoprotein hormones known as gonadotropins. Gonadotropins regulate normal growth, sexual development and reproductive function.1-2 This antibody detects syncytiotrophoblasts in placenta and in tumors such as choriocarcinoma and some cases of seminoma. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody-primary antibody-antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen.

【Main components】

Immunoglobulin, antibody diluent

【Storage】

Store at 2～8℃ for 18 months。

【Sample requirements】

FFPE tissues are usually cut into sections as thin as 3～5μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.

【Protocol】

1. Sample preparation：Deparaffinize the slides in xylene Ⅰ, Ⅱ, Ⅲ for 5 minutes；Transfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2. Blocking：Block endogenous peroxidase activity by incubating sections in 3% H₂O₂ solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3. Antigen retrieval：Heat the EDTA Antigen retrieval buffer to 100℃. Then place the slides in the boiled buffer and continue to heat for 15～20 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4. Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing.

5. 2차 항체 : 슬라이드를 비웁니다. 조직에 2 차 항체를 추가하고 실온에서 20 분 동안 배양 합니다 . PBST에서 슬라이드를 2회, 각 회당 5분 동안 세척합니다 .

6. DAB ：슬라이드를 비웁니다. 조직에 DAB를 추가 하고 실온에서 5분 동안 배양합니다. 탈이온수로 슬라이드를 헹굽니다.

7. 헤마톡실린 염색 ：슬라이드를 비운다. 조직에 헤마톡실린을 첨가하고 실온에서 5분 동안 배양 합니다 . 물로 슬라이드를 헹굽니다. 차별화를 위해 산 용액을 사용합니다 . 물로 슬라이드를 헹굽니다.

8. 탈수 : 슬라이드를 75% , 95% , 100% 알코올에 2 분간 탈수 시킨다 . 슬라이드를 말립니다. 마운팅 매체를 사용하여 커버슬립으로 염색된 조직을 덮습니다.