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면역조직화학용 CD44 항체 시약

면역조직화학용 CD44 항체 시약

IHC 항체 -- CD44


  • 양성 대조군:

    Tonsil
  • 셀룰러 현지화:

    Cytoplasm/cell membrane
  • 애플리케이션:

    IHC-P
  • 이차항체:

    iVision™
  • 사양/ml:

    1、3、6、0.2(Concentrated)

제품명

IHC 항체 -- CD44


포장 사양

암호 클론

명세서

AM0049

DF1485

0.1ml , 0.2ml , 1ml, 1.5ml , 3ml , 6ml , 11ml , 30ml

AR0496

SD391

0.1ml , 0.2ml , 1ml, 1.5ml , 3ml , 6ml , 11ml , 30ml


용도

연구용으로만 사용하십시오. CD44  항체 시약은  면역조직화학적 검출 시스템을 사용하여 FFPE 조직 절편에서 현미경으로 CD44를 정성적으로 식별하는 데 사용하기 위한 것입니다 .


원칙 _

IN, LHRMIC4, CDW44, HCELL로도 알려진 CD44는 세포-세포 상호작용, 세포 접착 및 이동에 관여하는 세포 표면 당단백질입니다.  항-CD44는 요로 상피의 비신생물성 변화로부터 요로 상피 암종을 감별하는데 유용할 수 있습니다. 1 항체 를 추가 하여 조직 절편에 항원 결합시킨 다음 HRP 표지된 2차 항체 결합 1차 항체를 사용하여 2 차 항체 -1 차 항체 - 항원 복합체를 형성합니다 .   When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen. 


Main components

Immunoglobulin, antibody diluent


Storage

Store at 2~8℃ for 18 months


Sample requirements

FFPE tissues are usually cut into sections as thin as 35μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.


Protocol

1. Sample preparationDeparaffinize the slides in xylene , , Ⅲ for 5 minutesTransfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2BlockingBlock endogenous peroxidase activity by incubating sections in 3% H2O2 solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3Antigen retrievalHeat the EDTA Antigen retrieval buffer to 100. Then place the slides in the boiled buffer and continue to heat for 1520 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing. 

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DABDrain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin stainingDrain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. DehydrateDehydrate the slides in 75%, 95%, 100% alcohols for 2 minutes. Dry the slides. Cover stained tissue with a coverslip using mounting medium.


Positive localization

1. Positive localization: membrane.

2. Positive control: tonsil.


Precautions

1. Please read the instruction carefully and become familiar with all components of the kit prior to use, Strictly follow the instruction during operation.

2. DO NOT use the kit or any kit component after their.

3. Only trained professionals can use this kit. Please wear suitable lab coat and disposable gloves while handling the reagents.

4. Avoid contact of skin, eyes and mucous membranes with the chemicals.

5. DO NOT pipet by mouth.

6. Unused reagents, used kit and waste must be disposed according to local regulations.


Manufacturer

Company Name: Xiamen Talent Biomedical Technology Co.,Ltd

Address: No.3rd and 4th Floors , Building B10, No. 2068 Wengjiao Road West, BioMedical Park, Haicang District, Xiamen City, 36100 China

Tel: +86 592 6315755            

E-mail: tlsw@talentbiomedical.com                

Website: www.talentbiomedical.com


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